DNA印迹法

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中文名称: DNA印迹法

英文名称: Southern blotting

词 性: 名词

中文解释:

由萨慎(E.M.Southern)于1975年建立。变性DNA从电泳凝胶通过毛细管作用转移到纤维素膜等固相介质上,然后进行DNA杂交的操作过程。

英文解释:

A technique used to locate and identify a DNA fragment containing a specific sequence; a mixture of fragments is separated by electrophoresis, transferred to a nitrocellulose sheet, hybridized to a radioactively labeled DNA probe complementary to the desired sequence, and visualized by autoradiography.