RNA印迹法

取自 食品百科全书

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09:11 2010年7月6日的修订版本

中文名称: RNA印迹法

英文名称: Northern blotting

词 性: 名词

中文解释:

RNA从电泳凝胶转移到固相介质(如尼龙膜等)上,然后与互补的核苷酸序列杂交的操作过程。

英文解释:

Analogous to Southern blotting, a technique in which a mixture of RNA fragments is separated by electrophoresis, transferred to a nitrocellulose sheet, hybridized to a radioactively labeled DNA probe complementary to the desired sequence, and visualized by autoradiography; the technique can therefore be used to locate and identify an RNA fragment containing a specific sequence.